Acoustic Wave Sparsely-Activated Localisation Microscopy (AWSALM).

Project – Acoustic Wave Sparsely-Activated Localisation Microscopy (AWSALM).

Ultrasound Localization Microscopy (ULM) provides very high spatiotemporal resolution, but the use of microbubbles requires low contrast agent concentrations, a long acquisition time, and gives little control over the spatial and temporal distribution of the microbubbles. Acoustic Wave Sparsely-Activated Localization Microscopy (AWSALM) and fast-AWSALM for in vivo super-resolution ultrasound imaging, offering contrast on demand and vascular selectivity with the use of nanodroplets. We have demonstrated the feasibility of fast and selective measurement of microvascular dynamics in vivo with subwavelength resolution using ultrasound and acoustically activatable nanodroplet contrast agents.

The deliberate activation of nanodroplets allows for spatially selective super-resolution imaging in vivo.